RNA

Part:BBa_K206031:Design

Designed by: Alex Ng   Group: iGEM09_British_Columbia   (2009-06-06)


Key 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

taR12 sequence from Isaacs et al. (1) with the following modifications: a. removal of last 6 nucleotides from the 3' end, to reduce probability of unwanted binding from complimentary scars b. changing of nucleotide 13 from G to U, to allow complementarity with the suffix scar to block an exposed rbs


Source

1) Isaacs, FJ, Dwyer, DJ, Ding, C, Pervouchine, DD, Cantor, CR, and Collins, JJ. Engineered riboregulators enable post-transcriptional control of gene expression. 2004. Nature Biotechnology 22(7):841-847.

References